Neutrophil Gelactinase-Associated Lipocalin (NGAL)/Lipocalin-2 ELISA |
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A Potential Biomarker for Predicting Acute Kidney Injury, Nephritis , coronary heart disease & diabetes |
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Alternative name:
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Adipocytokines and insulin resistance: the possible role of lipocalin-2, retinol binding protein-4, and adiponectin |
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| TLCN2 (or neutrophil gelatinase-associated lipocalin) is a recently identified adipokine that belongs to the superfamily of lipocalins (such as RBP4), which seems to affect glucose metabolism and insulin sensitivity . LCN2 protein has been implicated in diverse actions, such as apoptosis and innate immunity, and is expressed in several tissues, including neutrophils, liver, kidney, adipocytes, and macrophages . LCN2 is highly expressed in adipose tissue in vivo and in vitro, and its secretion is regulated by the activation of inflammation and infection . LCN2 mRNA is also elevated in the liver of ob/ob mice, and circulating LCN2 levels are increased in obese animals and human subjects with type 2 diabetes (5,8). In fact, expression of LCN2 is elevated by agents that promote insulin resistance and is reduced by thiazolidinediones . In one study, serum concentrations of LCN2 were positively associated with waist circumference, percent body fat, systolic blood pressure, fasting glucose and insulin concentrations, fasting triglycerides, and markers for chronic inflammation. All these results persisted significantly after adjustment for sex and age . In another study, serum LCN2 levels were significantly elevated in patients with coronary heart disease and were independently associated with atherosclerosis. | |
| Esteve E, Ricart W, Fernández-Real JM. Diabetes Care. 2009 Nov;32 Suppl 2:S362-7. | |
Lipocalin 2 is a selective modulator of peroxisome proliferator-activated receptor-γ activation and function in lipid homeostasis and energy expenditure |
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We have previously identified lipocalin 2 (Lcn2)
as a cytokine playing a critical role in the regulation of body fat
mass, lipid metabolism, and insulin resistance. Lcn2 deficiency
reduces PPARγ gene expression in adipocytes. In this study, we
investigated the role of Lcn2 in PPARγ activation and function via
assessing the insulin sensitization and fatty acid (FA) homeostasis of
PPARγ agonist in high-fat diet (HFD)-induced obesity in Lcn2−/−
mice. We found that rosiglitazone (Rosi) significantly improved
insulin sensitivity in Lcn2−/− mice as effectively
as in wild-type (WT) mice; unfed-state levels of blood glucose, free
FAs, and triglycerides (TGs) were significantly reduced after a 25-d
treatment of Rosi in Lcn2−/− mice. However, Rosi
action on fat deposition and FA homeostasis was altered; Rosi-induced
body weight and subcutaneous fat gain and liver lipid accumulation
were markedly lessened in Lcn2−/− mice. The
results of in vivo metabolic labeling showed that Rosi
markedly reduced de novo lipogenesis in adipose tissue of
Lcn2−/− mice. In brown adipose tissue (BAT), the
expression of the genes functioning in TG hydrolysis and mitochondrial
oxidation was up-regulated more in Lcn2−/− than in
WT mice. Most strikingly, Rosi stimulated significantly higher levels
of uncoupling protein-1 expression in BAT, and completely rescued cold
intolerance in Lcn2−/− mice. We demonstrate that
Lcn2 is a critical selective modulator of PPARγ activation and
function in lipid homeostasis and energy expenditure. |
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| Daozhong Jin et al. February 2011,The FASEB Journal vol. 25 no. 2 754-764 | |
Lipocalin-2 deficiency impairs thermogenesis and potentiates diet-induced insulin resistance in mice |
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| OBJECTIVE: Lipocalin (LCN) 2 belongs to the lipocalin subfamily of low-molecular mass-secreted proteins that bind small hydrophobic molecules. LCN2 has been recently characterized as an adipose-derived cytokine, and its expression is upregulated in adipose tissue in genetically obese rodents. The objective of this study was to investigate the role of LCN2 in diet-induced insulin resistance and metabolic homeostasis in vivo. RESEARCH DESIGN AND METHODS: Systemic insulin sensitivity, adaptive thermogenesis, and serum metabolic and lipid profile were assessed in LCN2-deficient mice fed a high-fat diet (HFD) or regular chow diet. RESULTS: The molecular disruption of LCN2 in mice resulted in significantly potentiated diet-induced obesity, dyslipidemia, fatty liver disease, and insulin resistance. LCN2(-/-) mice exhibit impaired adaptive thermogenesis and cold intolerance. Gene expression patterns in white and brown adipose tissue, liver, and muscle indicate that LCN2(-/-) mice have increased hepatic gluconeogenesis, decreased mitochondrial oxidative capacity, impaired lipid metabolism, and increased inflammatory state under the HFD condition. CONCLUSIONS: LCN2 has a novel role in adaptive thermoregulation and diet-induced insulin resistance. | |
| Guo H et al. Diabetes. 2010 Jun;59(6):1376-85. Epub 2010 Mar 23. | |
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Human
Neutrophil Gelactinase Associated Lipocalin (NGAL) ELISA Code No.: SK00233-01 Size: 96 T Price: $360.00 Standard Range:78-5000 pg/ml Sensitivity: 10 pg/ml Sample Type: serum, plasma Sample Volume: 100 uL Dilution Factor: 50-100 Intra CV: 4-6% Inter CV: 8-10% Protocol: PDF |
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Mouse/Rat Neutrophil Gelactinase Associated Lipocalin (NGAL) ELISA Code No.: SK00233-06 Size: 96 T Price: $360.00 Standard Range:1.6-1000 ng/ml Sensitivity: 8 ng/ml Sample Type: serum, plasma Sample Volume: 50 uL Dilution Factor: 4 for rat serum, 2 for mouse serum Intra CV: 4-6% Inter CV: 8-10% Protocol: PDF |
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Rat Neutrophil
Gelactinase Associated Lipocalin (NGAL) ELISA Code No.: SK00233-09 Size: 96 T Price: $360.00 Standard Range:0.78-50 ng/ml Sensitivity: 30 pg/ml Sample Type: serum, plasma Sample Volume: 100 uL Dilution Factor: Intra CV: 4-6% Inter CV: 8-10% Protocol: PDF |
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Human Lipocalin-1ELISA Code No.: SK00487-01 Size: 96 T Price: $360.00 Standard Range:31-2000 pg/ml Sensitivity: 10 pg/ml Sample Type: serum, plasma Sample Volume: 100 uL Intra CV: 4-6% Inter CV: 8-10% Protocol: PDF |
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Anti Human
NGAL IgG Code No.: A00233-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human NGAL Rec. Ab Type: Polyclonal Purification: Protein A Applications: E, IHC (1-2 ug/ml) Data Sheet: PDF |
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Anti Human NGAL Monoclonal Antibody Code No.: A00233-05-100 Size: 100 ug Price: $260.00 USD Host: Mouse Antigen: human NGAL Rec. Ab Type: Monoclonal Purification: Protein G Applications: ELISA Data Sheet: PDF |
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Anti Human NGAL Monoclonal Antibody Code No.: A00233-06-100 Size: 100 ug Price: $260.00 USD Host: Mouse Antigen: human NGAL Rec. Ab Type: Monoclonal Purification: Protein G Applications: ELISA Data Sheet: PDF |
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Anti Human NGAL Monoclonal Antibody Code No.: A00233-07-100 Size: 100 ug Price: $260.00 USD Host: Mouse Antigen: human NGAL Rec. Ab Type: Monoclonal Purification: Protein G Applications: ELISA Data Sheet: PDF |
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Anti Human NGAL Monoclonal Antibody Code No.: A00233-08-100 Size: 100 ug Price: $260.00 USD Host: Mouse Antigen: human NGAL Rec. Ab Type: Monoclonal Purification: Protein G Applications: ELISA Data Sheet: PDF |
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Human
NGAL Recombinant Code No.: 00233-01-100 Size: 100 ug Price: $360.00 USD Protein ID:P80188 Gene ID: 3934 MW: 19.6 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 95% Data Sheet: PDF |
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Rat NGAL Recombinant Code No.: 00233-02-100 Size: 100 ug Price: $360.00 USD Protein ID:P30152 Gene ID: 170496 MW: 22 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 95% Data Sheet: PDF |
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Anti
Rat
NGAL IgG Code No.: A00233-02-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: Rat NGAL Rec. Ab Type: Polyclonal Purification: Protein A Applications: E, IHC (1-2 ug/ml) Data Sheet: PDF |
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| Name | Code | Size | Price ($) |
| Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) ELISA Kit | SK00233-01 | 96 T | 360.00 |
| Rat/Mouse Neutrophil Gelactinase Associated Lipocalin (NGAL) ELISA Kit | SK00233-06 | 96 T | 360.00 |
| Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) ELISA Kit | SK00233-09 | 96 T | 360.00 |
| Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) ELISA Set | SK00233-07 |
960T |
590.00 |
| Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) ELISA Set | SK00233-17 |
480T |
360.00 |
| Lipocalin-1 (Human) ELISA Kit | 96 T |
360.00 |
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| Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Rec. | 100 ug |
360.00 |
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| Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human), Biotinylated | 00233-01-50B |
50 ug |
490.00 |
| Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) Rec | 00233-02-100 | 100 ug | 360.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) IgG | 100 ug |
220.00 |
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| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human)IgG, Biotinylated |
A00233-01-50B |
50 ug |
350.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-05-100 | 100 ug | 260.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-05-1000 | 1 mg | 390.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-06-100 | 100 ug | 260.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-06-1000 | 1 mg | 390.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-07-100 | 100 ug | 260.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-07-100 | 1 mg | 390.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-08-100 | 100 ug | 260.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) Monoclonal Antibody | A00233-08-1000 | 1 mg | 390.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) IgG | 100 ug |
220.00 |
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| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat)IgG, Biotinylated |
A00233-02-50B |
50 ug |
350.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) Monoclonal Antibody | A00233-09-100 | 100 ug | 260.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) Monoclonal Antibody | A00233-09-1000 | 1 mg | 490.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) Monoclonal Antibody | A00233-10-100 | 100 ug | 260.00 |
| Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) Monoclonal Antibody | A00233-10-1000 | 1 mg | 490.00 |