Cardiotrophin-like cytokine factor 1 ( CLCF1) ELISA, Recombinant, Antibody

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Human Cardiotrophin-like Cytokine Factor 1 (CLCF1/CLF1), Recombinant
Alternative name: B-cell-stimulating factor 3 (BSF3) Novel neurotrophin-1 (NNT-1) Gene Name CLF
CLCF1 is a secreted cytokine with B-cell stimulating capability. CLCF1 belongs to IL-6 superfamily. CLCF1 binds to and activates the ILST/gp130 receptor. It predominantly expressed in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver. CLCF1 and CTNF are two natural ligands for CTNF Receptor. Muation of CLF had been identified on cold-induced sweating syndrome type 2 (CISS2) patients.
Inactivation of cardiotrophin-like cytokine, a second ligand for ciliary neurotrophic factor receptor, leads to cold-induced sweating syndrome in a patient
Ciliary neurotrophic factor (CNTF) receptor controls a pathway supporting the differentiation and survival of a wide range of neural cell types during development and in adulthood. Cardiotrophin-like cytokine (CLC)-cytokine-like factor 1 (CLF) composite cytokine is a second ligand for the CNTF alpha-component receptor (CNTFRalpha). This composite cytokine is built on the structural model of IL-12, with a complex formed by a four-helix bundle type I cytokine, CLC (also referred to as CLCF1), bound to a soluble receptor subunit, CLF (also known as CRLF1). We have reported mutations in the chaperone soluble receptor CLF, causing cold-induced sweating syndrome (CISS). In this study, we studied the CLC-mutated alleles in a patient suffering from a similar disease. This patient was compound heterozygous for two different CLC mutations. The first allele was inactivated by a stop codon at position 107 (Y107X). In the second allele, a R197L mutation in the CLC-predicted binding site to the CNTFRalpha was detected. Functional analysis of the mutated protein revealed an incapacity for R197L CLC to bind to CNTFRalpha and activate the subsequent signaling events. Structural and docking interaction studies showed that the R197L substitution destabilized the contact site between CLC and CNTFRalpha.
Rousseau F, , et al.Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):10068-73. Epub 2006 Jun 16.
Cross-species functional analysis of cancer-associated fibroblasts identifies a critical role for CLCF1 and IL6 in non-small cell lung cancer in vivo
Cancer-associated fibroblasts (CAFs) have been reported to support tumor progression by a variety of mechanisms. However, their role in the progression of non-small cell lung cancer (NSCLC) remains poorly defined. In addition, the extent to which specific proteins secreted by CAFs contribute directly to tumor growth is unclear. To study the role of CAFs in NSCLC, a cross-species functional characterization of mouse and human lung CAFs was performed. CAFs supported the growth of lung cancer cells in vivo by secretion of soluble factors that directly stimulate the growth of tumor cells. Gene expression analysis comparing normal mouse lung fibroblasts (NFs) and mouse lung CAFs identified multiple genes that correlate with the CAF phenotype. A gene signature of secreted genes upregulated in CAFs was an independent marker of poor survival in NSCLC patients. This secreted gene signature was upregulated in NFs after long-term exposure to tumor cells, demonstrating that NFs are "educated" by tumor cells to acquire a CAF-like phenotype. Functional studies identified important roles for CLCF1-CNTFR and IL6-IL6R signaling, in promoting growth of NSCLC cells. This study identifies novel soluble factors contributing to the CAF protumorigenic phenotype in NSCLC and suggests new avenues for the development of therapeutic strategies.
Vicent S et al. Cancer Res. 2012 Sep 7. [Epub ahead of print]




	Human Cardiotrophin-like cytokine factor 1 ( CLCF1) ELISA KIt Code No.: SK00158-01 Size: 96 T Price: $460.00 USD Standard range: 1.56-100 ng/ml Sensitivity: 50 pg/ml Sample Type: serum, plasma Sample require: 100 uL per well Intra-CV:4-6% Inter-CV: 8-10% Protocol: PDF
	Human Cardiotrophin-like cytokine factor 1 ( CLCF1) Recombinant Code No.: 00158-01-100 Size: 100 ug Price: $360.00 USD Protein ID:Q9UBD9 Gene ID: 23529 MW:22.2 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 90% Data Sheet: PDF
	Anti Human CLCF1 IgG Code No.: A00158-01-100 Size: 100 ug Price: $260.00 USD Host:Rabbit Antigen: human CLCF1 Specificity: Human Antibody Type:Purified IgG Purification: Protein A affinity Application: E, IHC Data Sheet: PDF
More IL-6 superfamily and Adiponectin related Proteins
IL-6 CNTF CLCF1 gp130

elisa kIT

Name	Code No.	Size	Price ($)
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) ELISA KIT	SK00158-01	96 T	460.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec.	00158-01-50	50 ug	220.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec.	00158-01-100	100 ug	360.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec.	00158-01-1000	1000 ug	3200.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec. Biotinylated	00158-01-50B	50 ug	460.00
Anti CLCF1 (Human) IgG	A00158-01-100	100 ug	260.00
Anti CLCF1 (Human) Monoclonal Antibody	A00158-02-100	100 ug	350.00